Free-swimming planulae were found highly enriched for functions related to cilia and microtubules, and the drastic morphogenetic process undergone by the planula while establishing the future body of the polyp may be mediated by specifically expressed Wnt ligands. Transcript expression profiling yielded clusters of annotated transcripts with functions related to each specific life-cycle stage. A de novo transcriptome was assembled from Illumina RNA-Seq data generated from six stages throughout the Aurelia life cycle. Here we provide the first comprehensive view of the entire complex life of the Aurelia Red Sea strain by employing transcriptomic profiling of each stage from planula to mature medusa. The moon jellyfish Aurelia aurita is a widespread scyphozoan species that forms large seasonal blooms. Transcriptome profiling of the dynamic life cycle of the scypohozoan jellyfish Aurelia aurita.īrekhman, Vera Malik, Assaf Haas, Brian Sher, Noa Lotan, Tamar Both inhibitors disturbed medusa morphogenesis at the oral end, suggesting involvement of lysosomal hydrolases in strobilation. To inhibit AGA function, we administered the lysosomal acidification inhibitors, chloroquine or bafilomycin A1, to animals during strobilation. Quantitative RT-PCR analysis revealed that AGA expression increased during strobilation, and was then decreased in medusae. The genomic region of Aurelia AGA was approximately 9.8Â kb in length and contained 12 exons and 11 introns.

Similar to AGAs from other species, Aurelia AGA possessed an N-terminal signal peptide and potential N-glycosylation sites. To investigate the molecular mechanisms of strobilation, we screened for genes that are upregulated during strobilation using the differential display method and we identified aspartylglucosaminidase (AGA), which encodes a lysosomal hydrolase. Transition from polyp to medusa is called strobilation. The life cycle of the moon jellyfish, Aurelia aurita, alternates between a benthic asexual polyp stage and a planktonic sexual medusa (jellyfish) stage. Tsujita, Natsumi Kuwahara, Hiroyuki Koyama, Hiroki Yanaka, Noriyuki Arakawa, Kenji Kuniyoshi, Hisato Molecular characterization of aspartylglucosaminidase, a lysosomal hydrolase upregulated during strobilation in the moon jellyfish, Aurelia aurita. pacifica, jointly affects the distribution pattern of A. We conclude that physical forces are primarily responsible for offshore dispersion of A. 3 km off the shore, where jellyfish-eating Chrysaora pacifica medusae were prevalent. However, there was an abrupt decline in A. up to 9 km off the ports, and the pattern of age increase could be simulated by a simple diffusion model. Post-ephyra stages occurred primarily outside the ports, and their age increased gradually offshore, ca.

Almost exclusive occurrence of the ephyra stage in the ports demonstrated that their seeding polyps reside in the port enclosures, and liberated ephyrae are rapidly exported offshore by tidal water exchange. aurita after release from strobilating polyps, to offshore waters of northern Harima Nada (eutrophic eastern Inland Sea of Japan) from January to May 2010. We examined the spatiotemporal dispersion process in 6 ports of ephyrae of A. have been increasingly significant, particularly in human perturbed coastal waters, where numerous artificial constructions increase suitable habitat for polyp populations. Recurrent outbreaks of the common jellyfish Aurelia aurita s.l.

Makabe, Ryosuke Takeoka, Hidetaka Uye, Shin-ichi (Cnidaria: Scyphozoa) from port enclosures: Physical and biological factors Offshore dispersion of ephyrae and medusae of Aurelia aurita s.l.

The ephyra we need to go deeper